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This web site states the following, "Generating stable transgenic zebrafish lines has historically been laborious, for three reasons." We noticed that the web page also said " Building transgenesis constructs by conventional subcloning can be difficult, especially when using long coding or regulatory sequences." It also said " Injecting plasmid DNA yields mosaic transient expression and low germline transgenesis rates. The Tol2kit uses site-specific recombination-based cloning multisite Gateway technology. It includes a variety of widely-useful entry clones." The header had Main Page as the highest ranking optimized keyword. It is followed by Att site sequences, Basic Gateway principles, and Inserts and ends which isn't as ranked as highly as Main Page. The next words tol2kit.genetics.utah.edu uses is Invitrogen manual. List of entry and destination vectors was included and might not be viewed by search parsers.